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Identification and Functionality of Adult Mouse Hematopoietic Stem Cell Side Populations
21 Jun 2010: This application note demonstrates a protocol for the isolation of HSCs using a combination of cell surface markers that are unique to HSCs, in combination with Hoechst dye efflux and use of the 375-nm near UV laser option on the BD FACSAria II cell sorter. The data demonstrates that the SP cells are phenotypically c-kit+Sca-1+Lin– (KSL), showing that this strategy can be used for the isolation and enrichment of a rare and homogeneous population of potential LT-HSCs that have the ability to diff
BD Biosciences
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Recent articles: |
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Cytotoxicity in Cells: Easy Determination Using Lonza ViaLight Plus and ToxiLight BioAssays on the SpectraMax L Microplate Luminometer
07 Jun 2010: The Lonza ViaLight® Plus and ToxiLight® Bioluminescent Cytotoxicity Assays exploit the cell characteristics of ATP levels and adenylate kinase release respectively, as cytotoxicity measures and enable detection using bioluminescent methods. This application note demonstrates that the SpectraMax L Microplate Luminometer and SoftMax Pro Software from Molecular Devices combine well with both assays providing a fast and sensitive combination of technologies in the field of cell death.
Molecular Devices
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High-Throughput Low-Volume DNA Quantification and Analysis
03 Jun 2010: This application note details the very high speed at which the Thermo Scientific Multiskan GO can perform full DNA quantification and spectral analysis on large numbers of low volume (5 - 10 µl) samples. With the Multiskan® GO, measurement of full DNA spectra between 220-380 nm from 96 samples can be performed in less than three minutes, providing a spectral measuring capacity of over 2,000 samples per hour.
Thermo Fisher Scientific
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Turbidometric Growth Curve Measurements and Liquid Evaporation Studies with a Microplate Photometer
24 May 2010: The ability to monitor bacterial samples is important for food testing safety and analysis. This paper describes how a filter-based microplate photometer, Thermo Scientific Multiskan FC, can be used reliably and easily to measure turbidity of bacterial samples in antibiotic susceptibility studies. The growth curve measurements were performed on a 96-well microplate.
Thermo Fisher Scientific
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DNA Measurements using the TrayCell
17 May 2010: This application note demonstrates the TrayCell as a useful tool for measuring the absorbance of samples where there is limited sample available or the sample needs to be recovered after measurement without dilution. Concentration values obtained with the TrayCell are comparable to those obtained with standard 10mm path length cuvettes.
Jenway Ltd
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Detecting Barbiturates by Reversed Phase Liquid Chromatography
05 May 2010: Barbiturates are central nervous system depressants and they act in a similar manner to alcohol and slow down many areas of the brain. This application note presents a method of extracting barbiturates from urine and plasma samples by using Tox-Elut solid phase extraction columns and identification through UV/Vis absorbance spectrum comparisons with a reference library.
Cecil Instruments Limited
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Membrane Fluidity Measurements Using UV Fluorescence Polarization and the POLARstar Omega
21 Apr 2010: In this application note the POLARstar Omega microplate reader from BMG LABTECH was used to study membrane fluidity via fluorescence polarization in the UV range. The method allows for the monitoring of large scale alterations in membrane fluidity (such as phase transitions), as well as more subtle changes in lipid dynamics.
BMG LABTECH
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Novel Dual Detection Flash Instrument Improves Sample Purity and Productivity
26 Jan 2010: This application note demonstrates superior resolving power of the Reveleris™ system. Detecting non-UV active compounds improves sample purity and recovery while eliminating unnecessary post-purification steps.
Grace Davison Discovery Sciences
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Determination of Sample Loss on the Reveleris™ Flash Chromatography System
25 Jan 2010: This application demonstrates no sample loss from the fractions collected as detected by the ELSD detector. This shows that the split to the ELSD does not contribute to significant sample loss. The sophisticated proprietary fluidic and splitting design of the Reveleris™ system enables flash chromatographers to detect previously undetectable non-chromophoric compounds.
Grace Davison Discovery Sciences
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Reveleris Applications
25 Jan 2010: This application note discusses the applications and technology behind the Reveleris™ System. The system's detection technology provides advanced signal processing, recognizing peaks and collecting previously undetected compounds.
Grace Davison Discovery Sciences
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