 Quantitative Determination of Proteins
02 Jul 2008 Generally, the concentration of proteins is measured using UV-Vis spectrophotometers. The JASCO V-630 Bio is a UV/Vis spectrophotometer designed for biochemical analysis. The V-630 Bio is equipped with 6 quantitative calibration curves based on UV absorption spectrophotometry including the Lowry, Biuret, BCA, Bradford, and WST methods. The preferred method can be selected by reviewing the sample and range of the quantitation and existence of any possible contaminants. This application note explains how to create the calibration curves for the Lowry, Biuret and UV absorption analysis methods for which the methods can differ depending on each document.
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